Tuesday, 18 October 2011

PCR VS Vector Cloning

Vector cloning is best for preparing a large quantity of the desired gene, however if the DNA is impure, PCR will then be a quicker method as it does not require cells. PCR can make a lot of copies before vector cloning starts and less time consuming as you do not need to find the desired gene and then clone it. PCR can be used even there is only a small amount of DNA. However, errors will happen during DNA replication, the number of desired copies may not be satisfactory. Vector cloning is a more accurate method.



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