DNA Replication
DNA polymerase III catalyses the elongation of new DNA at a replication fork
DNA strands grows form 5’ to 3’ direction
Leading strand- copied into the direction of the fork continuously from a single primer
Lagging strand- copied away from the fork in short segments called Okazaki fragments, each requiring a new primer
DNA Transcription
Transcription: initiation, elongation and termination
Transcription factors recognizes the 5’ TATA 3’ box
Terminator sequence AAUAA
A 5’ cap is at the 5’ end of the pre-mRNA molecule
A poly(A) tail is at the 3’ end
mRNA is read from 5’ to 3’ codon by codon
The P site on ribosome holds the tRNA with the polypeptide chain
The A site has the tRNA with the next amino acid
It is at the E site where tRNA is discharged
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