Tuesday 18 October 2011

PCR VS DNA sequencing

PCR starts with a double stranded DNA but DNA sequencing starts with a single-stranded DNA. Both methods require DNA polymerase and nucleotides. PCR require primers but DNA sequencing require radioactively labeled primer and dideoxynucleotides that stop the event of elongation as they do not have a 3’-OH in the structure. PCR is a cycle but DNA sequencing is not. Different form PCR with repetitive heating, cooling and replication, DNA sequencing needs to undergo gel electrophoresis and autoradiography.


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